Guemei, A., Dessouky, E., Shalaby, T., Amer, S., Nassra, R. (2021). Study of the Efficacy and Multidrug Resistance Using Gold Nanoparticles-Based Drug Delivery Versus Conventional Chemotherapy in Non-Small-Cell Lung Cancer Cell Line. Research in Oncology, 17(2), 42-50. doi: 10.21608/resoncol.2021.70125.1134
Aida A. Guemei; Eman Dessouky; Thanaa I. Shalaby; Sarah K. Amer; Rasha Nassra. "Study of the Efficacy and Multidrug Resistance Using Gold Nanoparticles-Based Drug Delivery Versus Conventional Chemotherapy in Non-Small-Cell Lung Cancer Cell Line". Research in Oncology, 17, 2, 2021, 42-50. doi: 10.21608/resoncol.2021.70125.1134
Guemei, A., Dessouky, E., Shalaby, T., Amer, S., Nassra, R. (2021). 'Study of the Efficacy and Multidrug Resistance Using Gold Nanoparticles-Based Drug Delivery Versus Conventional Chemotherapy in Non-Small-Cell Lung Cancer Cell Line', Research in Oncology, 17(2), pp. 42-50. doi: 10.21608/resoncol.2021.70125.1134
Guemei, A., Dessouky, E., Shalaby, T., Amer, S., Nassra, R. Study of the Efficacy and Multidrug Resistance Using Gold Nanoparticles-Based Drug Delivery Versus Conventional Chemotherapy in Non-Small-Cell Lung Cancer Cell Line. Research in Oncology, 2021; 17(2): 42-50. doi: 10.21608/resoncol.2021.70125.1134
Study of the Efficacy and Multidrug Resistance Using Gold Nanoparticles-Based Drug Delivery Versus Conventional Chemotherapy in Non-Small-Cell Lung Cancer Cell Line
1Department of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
2Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt
3Medical Biophysics Department, Medical Research Institute, Alexandria University, Alexandria, Egypt
4Department of Pharmaceutics, Faculty of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, Alexandria, Egypt
Abstract
Background: Cisplatin is the main chemotherapy in the treatment of non-small-cell lung cancer (NSCLC) combined with gemcitabine or other agents. Aim: The aim of this study was to evaluate in vitro the anti-tumor efficacy and multidrug resistance of gold nanoparticles (GNPs) conjugated with cisplatin and /or gemcitabine for the treatment of NSCLC. Methods: MTT cytotoxicity assay was carried out in NSCLC cell line (A549). Cell viabilities were determined after treatment with different concentrations of cisplatin, gemcitabine, GNPs-cisplatin or GNPs-gemcitabine. Combinations of variable ratios (1:1, 1:2, 2:1, 1:9, 9:1) of cisplatin + gemcitabine, GNPs-cisplatin + gemcitabine, cisplatin + GNPs-gemcitabine or GNPs-cisplatin + GNPs-gemcitabine were also tested. The Combination Index was computed for each combination concentration ratio and dose to know the best synergistic effect. Histological changes and multidrug resistance were studied. Results: The 1:1 ratio combination concentration showed the highest synergistic effect among all concentration ratios (p < 0.001). Multidrug resistance revealed that the percent efflux of the GNPs-cisplatin + GNPs-gemcitabine combination with a 1:1 ratio was the minimal in comparison to the other drug combinations. Conclusion: The use of nanoparticles functionalized with cytotoxic drugs such as cisplatin or gemcitabine alone or in combination enhances their antitumor effect and decreases the multidrug resistance in NSCLC cell line.
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